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Malolactic fermentation (MLF) occurs in wine as the result of the metabolic activity of certain
adapted strains of lactic acid bacteria (LAB). The reduction of wine acidity and the modification of
wine flavour due to this secondary bacterial fermentation are often considered to benefit wine
quality. The advantages of induction of MLF by inoculation with selected strains of LAB are twofold.
First, to have better control over the time and speed of malic acid conversion, and, second, to have
a positive influence on wine flavour and quality. Sensory studies show that flavour compounds
produced by LAB impart recognizable changes to the flavour characteristics of the wine 1. Several
studies show that different strains of malolactic bacteria (MLB) will have different sensory effects in
wines but the influence of the timing of bacterial addition and the level of inoculation is not
well understood.